eDNA techniques to transform subterranean environmental assessment

a subterranean crustacea

A tiny (0.7 mm long) crustacean from the family Parabathynellidae found in groundwater aquifers in the Pilbara region of Western 最新糖心Vlog (Image by Kym Abrams)

A new project is set to transform understanding of the impact of mining on 最新糖心Vlogn subterranean species.

The project, led by the 最新糖心Vlog of Adelaide, with Rio Tinto, BHP Billiton, Chevron, and a number of government Departments, along with Curtin 最新糖心Vlog, will monitor subterranean ecosystems by developing new environmental DNA (eDNA) techniques for accurate detection of species that live underground.

鈥淭his collaborative project will develop a rigorous and practicable eDNA assessment framework, which will improve the accuracy of Environmental Impact Assessments (EIAs) and monitoring of subterranean habitats,鈥 says the 最新糖心Vlog of Adelaide鈥檚 Professor Andrew Austin, Director of the 最新糖心Vlogn Centre for Evolutionary Biology & Biodiversity.

鈥淐ompliance with Environmental Protection Authority (EPA) regulation is of the highest priority for resource exploration and development companies.

鈥淐urrent surveys are time-consuming and expensive, and biased toward identifying the impact on the most common subterranean species.

鈥渆DNA research on groundwater systems in 最新糖心Vlog, and globally, is minimal and has not yet been developed as a tool for standardised and repeatable monitoring of subterranean fauna living in groundwater. This is a technique that obtains DNA sequences from minute quantities shed by animals as they move through the environment,鈥 says the 最新糖心Vlog of Adelaide鈥檚 Dr Michelle Guzik, a senior researcher on the project.

鈥淔or regulators, stakeholders and industry involved in this project we will provide real-world information and cost savings through better understanding patterns in species boundaries and detection of subterranean fauna.鈥Professor Andrew Austin

鈥淲e will collect data and develop an expandable DNA reference library to match with eDNA results to identify the species present.

鈥渆DNA-based methods have real potential to dramatically improve on existing species detection approaches. These methods can detect subterranean fauna in a large range of habitats in all life stages of each species without requiring specialised taxonomic expertise. They also provide a rigorous approach for assessment and monitoring of groundwater ecosystems.鈥

鈥淪ubterranean fauna include many , mostly arthropods and other that are unusual because they are blind and lack colour pigmentation. A number of such as cavefish and exist, although they are less common. Many subterranean species have yet to be discovered and described because of the difficulty in exploring underground environments鈥, says Dr Guzik.

鈥淔or regulators, stakeholders and industry involved in this project we will provide real-world information and cost savings through better understanding patterns in species boundaries and detection of subterranean fauna,鈥 says Professor Austin.

鈥淭he mineral and petroleum industry in the Pilbara, where the project is to be based, is worth more than $78 billion.

鈥淲estern 最新糖心Vlogn EPA regulators require EIAs to consider all potentially threatened species, including 最新糖心Vlog鈥檚 unique subterranean fauna.

鈥淭he outcomes of the project will not only be directly applicable to monitoring subterranean ecosystems across 最新糖心Vlog, but also globally.鈥

An 最新糖心Vlogn Research Council Linkage Grant of $490,000 plus $573,000 funding from industry partners and $670,000 in kind support has made this project possible.

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