最新糖心Vlog

Dr Stuart Roy
Course Co-ordinator
Plant Genomics Centre, Rm 2.10
stuart.roy@acpfg.com.au


Dr Chad Habel
CLPD
Level 2 West, Schulz Building
North Terrace Campus
Tel: +61 8 8303 3019
chad.habel@adelaide.edu.au

1	Identify various 'omics approaches and their role in understangin plant function
2	Understand the differences between forward and reverse genetic approaches and how to make jugements when the different approaches are more appropriate
3	Analyse information from plant genomics research and recognise its potential in crop improvement
4	Design and develop experiments to understand and manipulate plant function
5	Evaluate the relative merits for particular situations of different genomic and genetic approaches for research and its application

In preparation for this course, students should read the following articles:

• Langridge P. & Fleury D. (2011) Making the most of ‘omics’ for crop breeding. Trends in Biotechnology, 29, 33-40.
• Rivandi, J. Miyazaki J., Hrmoca M., Pallotta M., Teser M., Collins N.C., (2011) A SOS3 homologue maps to HvNAX4, a barley locus controlling an environmentally sensitive Na⁺ exclusion trait. Journal of Experimental Botany, 62, 1201-1216
• Roy S.J., Tucker E.J. & Tester M. (2011) Genetic analysis of abiotic stress tolerance in crops. Current Opinion in Plant Biology, 14, 232-239.
• Roessner U. & Bowne J. (2009) What is metabolomics all about? BioTechniques, 46, 363-365.
• Roy S.J., Huang W., Wang X.J., Evrard A., Schmöckel S.M., Zafar Z.U. & Tester M. (2013) A novel protein kinase involved in Na⁺ exclusion revealed from positional cloning. Plant Cell & Environment, 36, 553-568
• Schilling RK, Marschner P, Shavrukov Y, Berger B, Tester M, Roy SJ, Plett DC (2013) Expression of the Arabidopsis vacuolar H⁺-pyrophosphatase gene (AVP1) improves the shoot biomass of transgenic barley and increases grain yield in a saline field Plant Biotechnology Journal DOI: 10.111/pbi.12145
• Shavrukov Y, Bovill J, Afzal I, Hayes JE, Roy SJ, Tester M, Collins NC (2013) HVP10 encoding V-PPase is a prime candidate for the barley HvNax3 sodium exclusion gene: evidence from fine mapping and expression analysis. Planta 237, 1111-1122

• Acquaah G (2007) Principles of plant genetics and breeding, Blackwell Publishing, Malden. 
• Buchanan B, Gruissem W, Jones R (2000) Biochemistry and molecular biology of plants. Rockville,Md.: American Society of Plant Physiologists. 
• Glick B (2003) Molecular biotechnology: principles and applications of recombinant DNA 
• Kole, C., Michler, C.H., Abbott, A.G & Hall, T.C. (eds.) 2010, Transgenic Crop Plants, Volume 1: Principles and Development, Springer-Verlag, Berlin.
• Kole, C., Michler, C.H., Abbott, A.G & Hall, T.C. (eds.) 2010, Transgenic Crop Plants, Volume 2: Utiliization and Biosafety, Springer-Verlag, Berlin.
• Taiz L, Zeiger E (2010) Plant physiology. Sunderland, Mass: Sinauer Associates
• Trigiano RN, Gray DJ (2005) Plant development and biotechnology

Writing guide: The following booklet provides a useful guide on written communication in science:

• Cargill, M. & Bellotti, M. 2004, Written Communication in the Agricultural and Natural Resource Sciences, The 最新糖心Vlog of Adelaide, Adelaide. http://www.agwine.adelaide.edu.au/students/external/carwripg1.pdf

Teaching materials and course documentation will be posted on the MyUni website ()

The course will include a series of lectures which will be complimented by practical sessions and problem-based group learning solutions. Students are encouraged to attend the weekly 最新糖心Vlogn Centre for Plant Functional Genomics seminars (Thursdays at 4pm) which will cover a range of topics and applications of the ‘omics technologies used in Plant Genomics. The seminars are given by national and international experts in the field of Plant Genomics.

A student enrolled in a 3 unit course, such as this, should expect to spend, on average 12 hours per week on the studies required. This includes both the formal contact time required to the course (e.g., lectures and practicals), as well as non-contact time (e.g., reading and revision).

Week	Lecture/Practical/Class exercise	Topic
Week1
	Practical 1 Monday 12th May 9.30am-1pm	Determining allelic diversity of salinity tolerance genes in barley (part 1) Stuart Roy
	Class exercise Monday 12th May 2-4pm
	Lecture 1 Tuesday 13th May 9.30-10.30am	Introduction to plant genomics Stuart Roy
	Lecture 2 Tuesday 13th May 10.30-11.30am	Forward and reverse genetics Stuart Roy
	Class exercise Wednesday 14th May 9-11am
	Practical 2 Wednesday 14th May 1-5pm	Determining allelic diversity of salinity tolerance genes in barley (part 2) Stuart Roy
	Lecture 3 Thursday 15th May 9.30-11.30am	Plant phenotyping and QTL mapping Stuart Roy
	ACPFG seminar
	Class exercise Friday 16th May 9-11am
	Lecture 4 Friday 16th May 1-3pm	Transcriptomics Ute Baumann
Week 2
	Practical 3 Monday 19th May 9.30am-1pm	Determining allelic diversity of salinity tolerance genes in barley (part 3) Stuart Roy
	Class exercise Monday 19th May 2-4pm
	Class exercise Tuesday 20th May 9-11am
	Lecture 5 Wednesday 21st May 9.30-11.30am	Metabolomics Ute Roessner
	Practical 4 Wednesday 21st May 1-5pm	Determining allelic diversity of salinity tolerance genes in barley(part 4)
	Lecture 6 Thursday 22nd May 9.30-11.30am	Generation of transgenic plants Darren Plett
	ACPFG seminar 4-5pm
	Class exercise Friday 23rd May 1-3pm
	Lecture 7 Friday 23rd May 1-3pm	Characterisation and functional analysis of transgenic plants Andrew Jacobs
Week 3
	Practical 5 Monday 26th May 9.30am-1pm	Determining allelic diversity of salinity tolerance genes in barley (part 5) Stuart Roy
	Class exercise Monday 26th May 2-4pm
	Lecture 8 Tuesday 27th May 9.30-11.30am	Case study 1: Positional cloning and characterisation of a novel protein kinase for plant salinity tolernace Stuart Roy
	Class exercise Wednesday 28th May 9-11am
	Practical 6 Wednesday 28th May 1-3pm	Revision
	Lecture 9 Thursday 29th May 9.30-11.30am	Case study 2: Expression of the Arabidopsis vacuolar H+ pyrophosphatase gene (AVP1) improves the shoot biomass of transgenic barley and increases grain yield in a saline field Stuart Roy
	ACPFG seminar
	Lecture 10 Friday 30th May 1-3pm	Revision
	Exam Tuesday 3rd June 9.30am-12.30pm

Practical session code of conduct

Practicals will be held in the teaching laboratories below the Charles Hawker Centre 

All students must follow these rules in practical exercises

1. No eating, drinking, application of makeup, etc. in laboratories; no water bottles or items of food or drink may be visible in laboratories

2. Closed shoes must be worn at all times in all practical exercises, wherever they are held.

3. Lab coats and other personal protective equipment must be worn at all times in laboratories or other areas whenever students are instructed to wear them; these should be removed when leaving the laboratory.

4. Bags, coats, etc. that are brought into a lab must be placed under the bench to avoid causing obstructions.

5. All containers used during a practical session must be labelled, including water and other common non-toxic substances

6. Report all accidents, both personal and/or involving experimental materials, to the person in charge

7. Playing around in a laboratory and any other work areas is dangerous; participants in improper behaviour will be asked to leave

8. Students must dispose of materials as directed

9. Students must wash their hands upon departing the labs and other work areas

10. Students must clean and tidy their bench and/or work area before departure; benches and/or work areas will be inspected at the end of each practical session. Place discarded cultures and infections material in the containers provided. Never discard cultures in any other place, especially not into the sinks

Assessment Task	Percentage of total assessment of grade	Learning Outcomes being assessed
Final Exam	40%	1,2,3,5
Poster project	30%	1,3,5
Short answer questions to practicals and practical report	30%	2,3,4

The Plant Biotechnology programs are delivered through face-to-face class sessions in which student interactions with the lecturer and fellow students are expected and encouraged. It is assumed that students will attend all scheduled classes. If you are absent, it is your responsibility to catch up on information discussed in class; do not expect the lecturer to repeat part of a class session to cover your absence. 

If you have not attended at least 80% of the class sessions, you will forego your right, on academic grounds, to any additional assessment opportunities. The course coordinator will monitor attendance. Whether it is assessed or not, it is important that you attend classes and contribute to discussions. Make sure that you complete any required preparatory work to be able to contribute effectively to class discussions and gain the most out of your education

Practical report: During the course of the practicals you will be asked to answer twelve questions relating to what you learned during that day in the practical the practical. Questions will be based your knowledge of various aspects of the techniques you used and the advantages and disadvantages of the type of experiment performed. After the very last practical you will be asked to write a short practical report  describing both your findings and the findings of the group.

Poster project: Students will begin the poster project in Foundations in Plant Biotechnology

Exam: The final examination will be a three hour short answer written examination, with questions designed to assess your understanding of the concepts covered in the course

Submission of assessments:

Practical assessment:
submission of answers to the practical questions will be through the MyUni site. Short questions will appear online after each practical for answering. The final practical report will also be submitted online through the MuUni site. Feedback from the lecturer will be at classes and through the MyUni site. All submissions must use the course cover-sheets provided in the course



Late submission of assessments:

If an extension is not applied for, or not granted then a penalty for late submission will apply. A penalty of 10% of the value of the assignment for each calendar day that is late (i.e. weekends count as 2 days), up to a maximum of 50% of the available marks will be applied. This means that an assignment that is 5 days or more late without an approved extension can only receive a maximum of 50% of the mark.